Next-generation sequencing refers to high-throughput sequencing technologies, including Illumina Sequencing, Life Technology semiconductor-based Ion Proton sequencing, Pacific BioSciences, Oxford Nanopore sequencing, and others.
What high-throughput sequencing platforms are available at Indiana University?
The Center for Medical Genomics at Indiana University School of Medicine is equipped with the following platforms:
Illumina NovaSeq 6000 (x2)
Illumina HiSeq 4000
Illumina NextSeq 500
LifeTech Ion Proton sequencing system
Oxford Nanopore MinION platform
What is the average turnaround time for service requests?
For most library preparation and sequencing projects, average turnaround time is four weeks. However, for projects with a large number of samples or experiments that involve non-standard protocols, the turnaround time may be longer. For sequencing of pre-made libraries, the average turnaround time is two weeks.
How long does the Center for Medical Genomics hold samples after a project is closed?
After the experiment is concluded, the Center for Medical Genomics stores the remaining samples for up to three months. Please contact the Center’s program manager to retrieve the remaining samples.
How should a user contact CMG for a quote or technical consultation for a project?
For all new users, an orientation meeting covers the logistics for using the Center’s services. This meeting should include the PI who can make fiscal and logistics decisions and technical staff who will directly prepare the samples.
How does the center assess the quality of the samples submitted?
Agilent Bioanalyzer 2100 or TapeStation 2200 is used to assess the DNA and RNA quality submitted by the users. For liability reasons, all samples are evaluated before the procedure, regardless of whether the users have evaluated in their own laboratories.
Can a user prepare their own library for sequencing?
Yes. The Center for Medical Genomics supports experiments with user-prepared library. However, the Center requires the users to share the full protocol for the library preparation and provide the exact specifications for sequencing. The Center is not fully responsible for the quality of the data if a large portion of the experiments are beyond the Center’s control. Please contact the Center’s program manager for material requirements for pre-made libraries.