Service | Comments | Single item cost ($) | Examples |
---|---|---|---|
Pulling and filing blocks (cases retrieved)/block consult | (PI must have IRB approval) | 15/case | |
Preparation of frozen blocks | Embedding in OCT | 5 | |
Preparation of paraffin blocks from wet tissue (each) | Includes processing and embedding in paraffin (IRB or IACUC required) | 25
|
|
More than 4 tissues in a cassette | 40 | ||
Decalcification (5% Formic acid) | Per tissue | 25 | |
Sectioning paraffin or frozen blocks (each slide) | Section before staining or unstained (add to above, if applicable) | 10/slide | |
More than two (2) levels or sections per slide | 15/slide | ||
Special needs: scrolls, thick sections (greater than 5mm), whole mounts, punches, etc | add 5/section | e.g., 10mm slides from paraffin block pulled from file:
($15 to pull)+ 10+5=$15 per slide or scroll |
|
H & E performed on blank slide | Add to above if applicable | 15 | e.g., from wet tissue to first H&E is $50/sample |
Routine special stain (e.g., PAS) performed on blank slide | Add to above if applicable | 50 | e.g., from wet tissue to first PAS is $85/sample |
Immunohistochemistry performed on blank slide
(Add to above if applicable) |
Antibody provided by IHC lab | 100 | e.g., from wet tissue to first antibody stain is 25+10+100 = $135/sample) |
Established antibody provided by researcher (dilution is known). | 90 | e.g., from wet tissue to first antibody stain is 25+10+90 = $125/sample | |
One time fee to titer new antibody provided by researcher. | 500 | “Bringing on new antibodies” is a huge consumption of time and there are no guarantees that the antibody will work on your tissue. | |
Special procedures performed on blank slide
(Add to above if applicable) |
Hercept Test In-Lab Kit | 200 | |
Hercept Test Researcher Kit | 90 | ||
Fluorescence stain | 100 | ||
Double-stain (IHC or Fluor) | 200 | ||
Slide boxes | Fisher plastic boxes (if necessary) | 15 | |
Spatial transcriptomics / slide Visium or Visium HD platform |
Includes oven incubation, desiccator Each additional hour of tech time = $200 Pick up slides after desiccation step |
$200 / slide | |
Custom studies (e.g., triple labels) | Negotiate w/ research director |