SERVICE | COMMENTS | Single item cost ($) | EXAMPLES |
Pulling and filing blocks (cases retrieved)/block consult | (PI must have IRB approval) | 15/case | |
Preparation of frozen blocks | Embedding in OCT | 2 | |
Preparation of paraffin blocks from wet tissue (each) | Includes processing and embedding in paraffin (IRB or IACUC required) | 15
|
|
More than 4 tissues in a cassette | 40 | ||
Decalcification (5% Formic acid) | Per tissue | 6.5 | |
Sectioning paraffin or frozen blocks (each slide) | Section before staining or unstained (add to above, if applicable) | 5/slide | |
More than two (2) levels or sections per slide | 10/slide | ||
Special needs: scrolls, thick sections (greater than 5mm), whole mounts, punches, etc | add 2/section | e.g., 10mm slides from paraffin block pulled from file:
($15 to pull)+ 5+2=$7 per slide or scroll |
|
H & E performed on blank slide | Add to above if applicable | 8 | e.g., from wet tissue to first H&E is $28/sample |
Routine special stain (e.g., PAS) performed on blank slide | Add to above if applicable | 10 | e.g., from wet tissue to first PAS is 15+5+10 = $30/sample |
Immunohistochemistry performed on blank slide
(Add to above if applicable) |
Antibody provided by IHC lab | 25 | e.g., from wet tissue to first antibody stain is 15+5+25 = $45/sample) |
Established antibody provided by researcher (dilution is known). | 20 | e.g., from wet tissue to first antibody stain is 15+5+20 = $40/sample | |
One time fee to titer new antibody provided by researcher. | 150 | “Bringing on new antibodies” is a huge consumption of time and there are no guarantees that the antibody will work on your tissue. | |
Special procedures performed on blank slide
(Add to above if applicable) |
Hercept Test In-Lab Kit | 50 | |
Hercept Test Researcher Kit | 20 | ||
Fluorescence stain | 25 | ||
Double-stain (IHC or Fluor) | 50 | ||
Slide boxes | Fisher plastic boxes (if necessary) | 15 | |
Custom studies (e.g., triple labels) | Negotiate w/ research director |