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Center for Electron Microscopy

The Indiana University School of Medicine Center for Electron Microscopy is a full-service research laboratory that offers cryo-electron microscopy, transmission electron microscopy, and scanning electron microscopy. The core can provide technical services to help design and then implement experiments needing each type of microscopy.

Consultations are required for new projects for a fee of $100. This fee is refunded to the user after project completion. The service provided can apply both traditional methods and more recent technical developments to meet the investigator’s needs.

Full Service Support

The following procedures are provided as a full service with independent use allowed for use on the microscopes (TEM, Transmission Electron Microscope and Cryo-EM, Cryo-Electron Microscopy). Training is provided at the full-service price until capabilities on the equipment are confirmed. Independent work is billed at a lower cost (see fee schedule).

The core director has the authority to revoke the independent-use privilege or require retraining to ensure the safety of the equipment. All additional training requested or required are billed at the technician rate.

Pricing

Pricing for services provided by this core is available on the Fee Schedule.

Equipment

a large enclosed microscope with a digital screen on the front. the bottom corner reads "Glacios"

Glacios Cryo-TEM 

ThermoFisher Glacios Cryo-TEM equipped with Falcon 4 detector

the titan krios microscope is a futuristic white paneled box with a large black door

Titan Krios G4 Cryo-TEM

ThermoFisher Titan Krios G4 Cryo-TEM (housed at Purdue as part of local consortium)

the vitrobot mark IV system

Vitrobot Mark IV System

The Vitrobot Mark IV System automates the vitrification process to provide fast, easy, reproducible sample preparation.

TEM Microscope

Tecnai Spirit. ThermoFisher, Hillsboro, OR equipped with AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA)

the PELCO easiGlow instrument

PELCO easiGlow

Designed for cleaning TEM grids and hydrophilization of TEM carbon support films

Ultramicrotomes

UCT Ultracut with FCS. FCS, Cryosectioning Attachment (Leica, Bannockburn, IL)

Sputter Coater

Polaron (Energy Beam Sciences, East Gramby, CT)

Vibratome

Tecnai Spirit. ThermoFisher, Hillsboro, OR equipped with AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA)