Wilms Tumor 1 (WT1) Expression Analysis

CPT Code(s): 81479

Ordering Recommendation: Monitoring of Wilms tumor 1 (WT1) expression in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) patients to aid clinicians in determining follow up procedures and therapeutic strategies.

Synonyms: Gene expression, quantitative RT-PCR, quantitative real-time PCR, polymerase chain reaction, acute lymphoblastic leukemia (ALL), acute myeloid leukemia (AML), Wilms tumor 1 gene, WT1 

Methodology: Reverse transcription quantitative real-time PCR (RT-qPCR).

Performed: Weekly

Reported: 7-10 days

Collect: Lavender (EDTA) tubes.

Specimen Volume: 1-3 mL of whole blood/bone marrow.

Storage/Transport: Refrigerated or room temperature. Specimen should arrive in laboratory ≤24 hours after collection.

Unacceptable Conditions: Frozen, grossly hemolyzed, clotted, or arrives in the laboratory ≥48 hours after collection.

Stability: 48 hours at 2°C to 8°C; one week at 2°C to 8°C after sample is mixed with 2X DNA/RNA Shield.