PML/RARA RNA Expression Analysis

CPT Code(s): 81315, 81316

Service Code (IU Health): 53053054

Ordering Recommendation: Diagnosis and monitoring of t(15;17) PML/RARA to aid clinicians in determining follow up procedures and therapeutic strategies for APL.

Synonyms: Acute promyelocytic leukemia, t(15;17), PML/RARA, bcr1, bcr2, bcr3, quantitative real-time PCR, qualitative RT-PCR, quantitative RT-PCR, polymerase chain reaction, gene expression

Methodology: Reverse transcription quantitative real-time PCR (RT-qPCR)

Performed: Weekly

Reported: 7-10 days

Collect: Lavender (EDTA) tubes

Specimen Volume: 1-3 mL of whole blood/bone marrow

Storage/Transport: Refrigerated or room temperature. Specimen should arrive in the laboratory less than or about 24 hours after collection.

Unacceptable Conditions: Frozen, grossly hemolyzed, clotted, or arrives in the laboratory 48 hours or more after collection.

Stability: 48 hours at 2°C to 8°C; one week at 2°C to 8°C after sample is mixed with 2X DNA/RNA Shield