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Myotonic Dystrophy Type 1, DMPK PCR

CPT Code(s):  81403

Service Code (IU Health): 53025516

Ordering Recommendation: DMPK mutation analysis is recommended for an individual with a clinical diagnosis of myotonic dystrophy type 1(DM-1); Carrier identification in individuals with a positive family history of DM-1.

Synonyms: DM-1 mutation analysis, DMPK CTG genotyping, CTG repeats

Methodology: Triplet Repeat Primed PCR (TP-PCR) and capillary electrophoresis. Southern analysis is performed as a send-out test, as needed, to further characterize expanded/abnormal alleles.

Performed: Monday through Friday

Reported: 6-9 days

Specimen Requirements

Collect: Lavender (EDTA) tubes; buccal swab; DNA

Specimen Volume: Blood: 2-6 mL whole blood; Buccal swab (Lab provides the collection tube)

Storage/Transport: Refrigerated/Room temperature

Unacceptable Conditions: Grossly hemolyzed or clotted

Stability: Two weeks refrigerated; One month frozen