Lymphomas — FISH Analysis

CPT Code(s):  88237, 88275, 88271 (x per probe)

Service Code (IU Health): 53101259, 53100707, 53100640

Ordering Recommendation: For detection of a neoplastic clone that is observed in patients with lymphoma, to identify and track chromosome abnormalities in patients, and to evaluate response to therapy.

Synonyms: FISH, fluorescence in situ hybridization, hematopoietic malignancy, lymphoma, Hodgkin’s lymphoma, non- Hodgkin’s lymphoma, Burkitt Lymphoma, Burkitt-like Lymphoma, Diffuse Large Cell Lymphoma, Follicular Lymphoma, MALT Lymphoma, Mantle Cell Lymphoma, MYC - 8q24 Rearrangement

Methodology: Fluorescence in situ hybridization (FISH) analysis.

Performed: Monday through Friday

Reported: 2-3 days

Panels: Lymphoma Panel - t(8;14)(MYC/IGH), MYC, t(14;18) (IGH/BCL2)

Collect: Non-diluted bone marrow aspirate in green-top sodium heparin tube, heparinized syringe.

Specimen Volume: Preferred: 2 mL for normal WBC (decreased WBC requires more, and elevated WBC requires less); Minimum: 0.5 mL

Storage/Transport: Room temperature. Do not freeze or expose to extreme temperatures.

Unacceptable Conditions: Frozen specimens. Formalin-fixed specimens.

Stability: Ambient: 48 hours; Refrigerated: 48 hours; Frozen: Unacceptable