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KIT Targeted Sequencing Analysis

CPT Code(s): 81272

Ordering Recommendation: Targeted KIT sequencing is recommended for individuals with acute myeloid leukemia with either t(8;21) or inv(16) to help determine prognosis.

Synonyms: KIT, exons 8 and 17, AML, acute myeloid leukemia, KIT proto-oncogene, receptor tyrosine kinase, CBF-AML, Core binding factor acute myeloid leukemia.

Methodology: Sanger sequence analysis. The KIT cDNA reference sequence used is NM_000222.2.

Performed: Monday through Friday

Reported: 14 days

Specimen Requirements

Collect: Preferred: whole blood in a lavender top (EDTA) tubes, cultured and uncultured cells

Specimen Volume: Blood: 3 mL whole blood (minimum 1 mL)

Storage/Transport: Refrigerated/Room temperature

Unacceptable Conditions: Grossly hemolyzed or clotted

Stability: One month refrigerated; One month frozen