Pelus Lab

The research lab of Louis M. Pelus, PhD focuses on understanding the regulation of survival proliferation and differentiation of normal adult blood and mesenchymal stem cells, how they respond to stress, and how this knowledge can be leveraged for therapeutic application in the areas of bone marrow transplantation, gene therapy and protection and mitigation of radiation injury.

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Active Research

The laboratory has identified GRO beta, a member of a family of peptides called chemokines, which stimulates the migration of stem cells that are able to completely reconstitute hematopoiesis from bone marrow to blood.

Studies are currently underway to determine the differences between GRO beta and G-CSF mobilized stem cells, the intracellular and extracellular events initiated by GRO beta -CXCR2 interaction that leads to migration of stem cells and how this is regulated, and how combination therapy with other cytokines, chemokines and their receptors can define new paradigms for stem cell transplantation. The answer to these questions will benefit patients receiving extensive chemotherapy who require hematopoietic support or who could benefit from curative hematopoietic stem cell transplantation.

Research Funding

  • PR140433, Pelus (PI), 10/1/2015-9/30/2018, Dept. of Defense

    Using the prostaglandin signaling cascade to mitigate the effects of acute and delayed radiation exposure to hematopoiesis and blood cell function.

    The objectives of this proposal are to investigate the ability of dmPGE2 to protect and mitigate the effects of radiation exposure on hematopoiesis and multi-organ dysfunction

  • 1R01 HL096305-06 Pelus (Role: PI), 9/1/2015 – 8/31/2019, NHLBI

    Role of PGE2 and other eicosanoids in hematopoietic stem cell function.

    This project will define the roles of EP4 signaling in the hematopoietic niche that regulate normal and stressed hematopoiesis is.

  • 1R01AG046246-01, Pelus Co-PI (with M. Kacena), 2/1/2013-1/31/2018, NIA

    A megakaryocyte/PGE2/osteoblast triad in the normal and aged niche.

    This project will define the regulatory interactions of PGE2, megakaryocytes, bone forming osteoblasts and mesenchymal stem cells in normal young and aged mice at homeostasis and after irradiation.

  • 1R01 CA182947-01, Pelus Co-PI (with S. Farag), 5/1/2014-4/30/2019

    NCI Inhibition of PGE2 for mobilization of autologous peripheral blood stem cells.

    The objectives of this proposal are to run a phase II clinical trial to assess the safety and efficacy of NSAIDs with G-CSF for autologous mobilization in patients with MM and NHL and molecularly characterize the NSAID assisted graft.

  • PR140896 Pelus Co-I (Orschell PI), 10/1/2015-9/30/2018, Dept. of Defense

    Mitigation of the Hematopoietic and Gastrointestinal Acute and Delayed Radiation Syndromes Using Combination Therapy with Pegylated Hematopoietic Growth Factors

  • 2T32 DK007519-26, Pelus (Co-PI with H. Broxmeyer), 7/1/1985 – 6/30/2021, NIH/NIDDK

    Regulation of Hematopoietic Cell Production. This is a training grant.

  • 1R01AG046246, Pelus Co-PI (with C. Orschell), 2016-2017, NIA

    Sex/gender differences in hematopoietic function and the stem cell niche.

Recent Publications

  • 2017
    P Singh, S Fukuda, LM Pelus. Survivin is required for mouse and human mesenchymal stem cell proliferation, survival and differentiation. Stem Cells, In Press 2017.

    AM Patterson and LM. Pelus. G-CSF in stem cell mobilization: new insights, new questions. Annals of Blood, In Press 2017.

    P Singh, J Hoggatt, M Kamocka, KS Mohammad, MR Saunders, H Li, JM Speth, N Carlesso, T Guise, LM Pelus.  Enzymatic cleavage of neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Investigation, In Press, 2017.

    J Hoggatt, P Singh, TA. Tate, B-K Chou, S Datari, S Fukuda, L Liu, PV Kharchenko, A Schajnovitz, N Baryawno, F Mercier, J Boyer, D Morrow, DT Scadden, Louis M. Pelus. Rapid mobilization reveals a highly engraftable hematopoietic stem cell. Cell, In Press, 2017.

  • 2015
    S Fukuda, J Hoggatt, P Singh, M Abe, JM Speth, P Hu, EM Conway, G Nucifora, S Yamaguchi, LM Pelus. Survivin modulates genes with divergent functions and regulates proliferation of hematopoietic stem cells through Evi-1. Leukemia 29:433-440, 2015. [PMID: 24903482].

    A Balogh , Y Shimizu , SC Lee, R Gangwar, DD Norman, M Bavaria, CS Moon, P Shukla, R Rao, R Ray, AP Naren, S Banerje, DD Miller, L Balazs, LM  Pelus, G Tigyi. The autotaxin-LPA2 GPCR axis is modulated by g-irradiation and facilitates DNA damage repair. Cell Signal Sep 27 9: 1751-1762, 2015. [PMID:  26027517].

  • 2014
    HE Broxmeyer, LM Pelus. Inhibition of DPP4/CD26 and dmPGE2 treatment enhances engraftment of mouse bone marrow hematopoietic stem cells. Blood Cells Mol Dis, 2014 Mar3, 53:34-38 pii: S1079-9796(14)00008-4. doi: 10.1016/j.bcmd.2014.02.002.  [PMID:24602918].
  • 2013
    J Hoggatt, KS Mohammad, P Singh, AF Hoggatt, BR Chitteti, JM Speth, P Hu, BA Poteat, KN Stilger, F Ferraro, L Silberstein, FK Wong, SS Farag, M Czader, GL Milne, RM Breyer, CH Serezani, DT Scadden, TA Guise, EF Srour, LM Pelus. Differential stem- and progenitor-cell trafficking by prostaglandin E2. Nature, 2013, 21;495(7441):365-9, doi:1038/nature11929. Epub 2013 Mar 13 [PMCID: PMC3606692].

    J Hoggatt, P Singh, KN Stilger, PA Plett, CH Sampson, HL Chua, CM Orschell, LM Pelus. Recovery from hematopoietic injury by modulating prostaglandin E(2) signaling post-irradiation. Blood Cells Mol Dis, 2013 Mar;50(3):147-53, doi:10.1016/j.bcmd.2012.11.006. [PMID:23206586].

    C Cutler, P Multani, D Robbins, HT Kim, T Le, J Hoggatt, LM Pelus, et al. Prostaglandin-modulated umbilical cord blood hematopoietic stem cell transplantation. Blood, Blood. 2013 Oct 24;122(17):3074-81. doi: 10.1182/blood-2013-05-503177. Epub 2013 Aug 30. [PMID:23996087].

    J Hoggatt, K Mohammed, P Singh and LM Pelus. Prostaglandin E2 enhances long-term repopulation but does not permanently alter inherent stem cell competitiveness. Blood, 2013 Oct 24;122(17):2997-3000. doi: 10.1182/blood-2013-07-515288. Epub 2013 Sep18. [PMID:24047650].

    JM Speth, J Hoggatt, P Singh, LM Pelus. Pharmacologic increase in HIF1 enhances hematopoietic stem and progenitor homing and engraftment. Blood, 2014, Jan 9;123(2):203-207. Doi:10.1182/blood-2013-07-516336.  2013 Oct 28. [Epub ahead of print] [PMID:24167196].

Faculty Research Team

Louis M. Pelus, PhD

Senior Research Professor of Medicine

Pratibha Singh, PhD, MSC

Assistant Professor of Pediatrics

Additional Research Team Members

Other research team members in the Pelus Lab include Liqiong Liu, PhD (Post-doctoral Fellow) and Andrea Patterson (Post-doctoral Fellow).