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Proteomics Core Facility

The Proteomics Core at Indiana University School of Medicine provides protein and proteomic analysis services for protein identification, characterization, and quantification. The core encourages collaborations that apply the tools of proteomics to cutting-edge biomedical research.

The Proteomics Core's vision is to deliver value to the scientific community as a world-class proteomics core lab providing services and collaborations to meet the critical needs of biomedical researchers in life sciences.

The core's mission is to provide analytical resources for qualitative and quantitative analysis of macromolecules and to foster critical collaborations in proteomics and biomarker development.

Services

  • Consult with Core Staff
    Help designing proteomics experiments and grant support letters.
  • Affinity Purification (Complex Protein Identification)
    Global identification of proteins in a sample. This may include peptide fractionation to increase proteome coverage. De novo sequencing and analysis of proteins from unsequenced organisms are available.
  • In-gel Digestion/Recombinant Protein Analysis (Simple Protein Identification)

    Identification of proteins from a gel band, immunoprecipitation, or affinity purification.

    *Biological duplicate recommended for publication*

  • Global Protein Quantification

    Tandem mass tag labeling, fractionation, and quantification of proteins from cell culture or tissue

    *Biological triplicate necessary for statistics*

  • Phosphorylation Quantification

    Phosphopeptide enrichment, tandem mass tag labeling, fractionation, and quantification of phosphopeptides from cell culture or tissue.

    *Biological triplicate necessary for statistics*

  • CETSA/TPP
    Cellular Thermal Shift Assay/Thermal Proteome Profiling (CETSA/TPP), an assessment of protein thermal stability. Data analysis, data consultation, re-analysis from a previous study or from data on ProteomeXchange.
  • Publication Support
    Extra figures, data analysis, writing consultation, and upload of all project data to a publically available source for publication submission

Sample Submission

All proteomics or mass spec samples should be submitted directly to the Proteomics Core labs. Outside users can submit their samples via express delivery only after proper handling is insured.

Shipping Address

635 Barnhill Drive
MS 0034
Indianapolis, IN 46202

All services are based on a first-come-first-served policy.

Download the Sample Submission Form