Bonewald Lab

The research lab of Lynda Bonewald, PhD, focuses on the biology and function of the osteocyte.

Investigators in this lab have two major focus areas: the crosstalk between osteocyte and muscle and the role of the osteocyte in calcium homeostasis under calcium demanding conditions.

Active Research

Osteocyte Regulation of Bone Muscle with Age

Muscle secretes factors that maintain osteocyte viability and function, and conversely osteocytes produce factors that support myogenesis and muscle function. These factors are being identified and their functions characterized.

Extracellular Vesicle Mediated Cell-Cell Communication in Bone

The lab is exploring the role of the osteocyte in calcium homeostasis under calcium demanding conditions such as during pregnancy. The mechanisms by which osteocytes can remove and replace calcium in their microenvironment is being examined. Trainees can learn osteocyte and muscle cell culture, isolation of primary osteocytes and muscle satellite cells, muscle contractility, application of fluid flow shear stress, mitochondrial imaging, loading and unloading of transgenic animals, in addition to standard molecular approaches and analyses.

  • Research Funding

    Osteocyte Regulation of Bone Muscle with Age- Core A: Administrative Core and Subproject I: Muscle Regulation of Osteoblast/Osteocyte Function in Young compared with Aged Animals. Extracellular Vesicle Mediated Cell-Cell Communication in Bone.

  • Recent Publications
    -Pacicca DM, Brown T, Watkins D, Kover K, Yan Y, Prideaux M, Bonewald L Elevated glucose acts directly on osteocytes to increase sclerostin expression in diabetes. Sci Rep 2019 PMID: 31757981.

    -Sego TJ, Prideaux M, Sterner J, McCarthy BP, Li P, Bonewald LF, Ekser B, Tovar A, Smith JL. Computational fluid Dynamic Analysis of Bioprinted Self-Supporting Perfused (SSuPer) Tissue Models. Biotechnol Bioeng. 2019 PMID 31788785.

    -Essex AL, Pin F, Huot JR, Bonewald LF, Plotkin LI, Bonetto A. Bishosphonate Treatment Ameliorates Chemotherapy-Induced Bone and Muscle Abnormalities in Young Mice. Front Endocrinol (Lausanne) 2019 PMID 31803146.

    -Mo C, Wang Z, Bonewald L, Brotto M. Multi-Staged Regulation of Lipid Signaling Mediators during Myogenesis by COX-1/2 Pathways. Int J Mol Sci, 2019 PMID 31579816.

    -Wang Z, Bian L, Mo C, Shen H, Zhao LF, Su KJ, Kukula M, Lee JT, Armstrong DW, Recker R, Lappe J, Bonewald LF, Deng HW, Brotto M Quantification of aminobutyric acids and their clinical applications as biomarkers for osteoporosis. Commun Bio 2020 PMID 31969651.

    -Wang H, Li T, Wang X, Yin X, Zhao N, Zou S, Duan P, Bonewald LF.  The role of sphingosine-1-phosphate signaling pathway in cementocyte mechanotransduction. Biochem BIophys Res Commun 2020 PMID 31941604.

    -Prideaux M, Kitase Y, Kimble M, O’Connell TM, Bonewald LF Taurine, an osteocyte metabolite, protects against oxidative stress-induced cell death and decreases inhibitors of the Wnt/b-catenin signaling pathway Bone 2020 PMIC 32330695.

    -Ito N, Prideaux M, Wijenayaka AB, Yang D, Ormsby RT, Bonewald LF, Atkins GJ Sclerostin directly stimulates osteocyte synthesis of Fibroblast Growth Factor 23 Cal Tiss Int. 2021 PMID33616712.

    -Pin F, Prideaux M, Huot JR, Essex AL, Plotkin LI, Bonetto A, Bonewald LF  Non-bone metastatic cancers promote osteocyte-induced bone destruction Cancer Lett 2021 PMIC 32233150. DOI:10.1016/j.canlet.2021.06.030

    -Nakamura MT, Zhang H, Guo D, Ueharu H, Pan H, Scott G, Harris M, Ray M, Feng JQ, Harris SE, Bonewald LF, Mishina Y Podoplanin is dispensable for mineralized tissue formation and maintenance in the Swiss outbred mouse background. genesis The Journal of Genetics and Development.  2021, https://doi.org/10.1002/dvg.23450

    For a full list of Dr. Bonewald’s publication history, please find her on Google Scholar (https://scholar.google.com/citations?user=FNdjsE0AAAAJ&hl=en) or PubMed (https://www.ncbi.nlm.nih.gov/pubmed/?term=bonewald%2C+l)

     

Faculty Research Team

Lynda F. Bonewald, PhD

Director, Indiana Center for Musculoskeletal Health

Matt Prideaux, PhD

Assistant Research Professor of Anatomy, Cell Biology & Physiology

Yukiko Kitase, PhD

Assistant Research Professor of Anatomy, Cell Biology & Physiology

Fabrizio Pin, PhD, MS

Assistant Scientist in Anatomy, Cell Biology & Physiology