1986 PhD State University of New York at Stony Brook

1976 AB University of California, Santa Cruz

Our laboratory studies endocytic membrane transport in polarized epithelial cells. The function of an epithelium depends upon the polar distribution of transporters and receptors on either the lumenal or basal side of the monolayer. We have shown that endocytosis is critically important to this polarity as the apical and basolateral membranes of epithelial cells are continuously and thoroughly intermixed in endosomes where proteins are sorted for return to the proper domain of the plasma membrane. We have developed powerful methods of quantitative confocal microscopy to address membrane transport in cells labeled with fluorescent conjugates of endocytic ligands and expressing GFP chimeras of endocytic regulatory proteins. The functions of regulatory proteins are evaluated through the expression of mutant proteins, and through novel methods of 3-dimensional, multi-parameter microscopy of living cells. This approach allows us to correlate defined steps of endocytic transport with the dynamic bahaviors of specific regulators. More recently our laboratory has begun using biochemical analyses of endosomes isolated by subcellular fractionation to identify proteins that regulate polar sorting in endosomes. Our laboratory has open access to the latest equipment for 3-dimensional microscopy, including four confocal microscopes, two multi-photon microscopes and an iterative deconvolution microscope system. We are also actively involved in developing advanced methods of microscopy and image processing. The equipment and research activities of our imaging facility are described at: Indiana Center for Biological Microscopy

Spatial Temporal Analysis of Fieldwise Flow in Microvasculature.
Clendenon SG; Fu X; Von Hoene RA; Clendenon JL; Sluka JP; Winfree S; Mang H; Martinez M; Filson A; Klaunig JE; Glazier JA; Dunn KW; Journal of visualized experiments : JoVE 2019 Nov 18

A Versatile, Portable Intravital Microscopy Platform for Studying Beta-cell Biology In Vivo.
Reissaus CA; Piñeros AR; Twigg AN; Orr KS; Conteh AM; Martinez MM; Kamocka MM; Day RN; Tersey SA; Mirmira RG; Dunn KW; Linnemann AK; Scientific reports 2019 Jun 11

DeepSynth: Three-dimensional nuclear segmentation of biological images using neural networks trained with synthetic data.
Dunn KW; Fu C; Ho DJ; Lee S; Han S; Salama P; Delp EJ; Scientific reports 2019 Dec 4

Quantitative Kinetic Models from Intravital Microscopy: A Case Study Using Hepatic Transport.
Tavakoli M; Tsekouras K; Day R; Dunn KW; Pressé S; The journal of physical chemistry. B 2019 Aug 15

Modeling of xenobiotic transport and metabolism in virtual hepatic lobule models.
Fu X; Sluka JP; Clendenon SG; Dunn KW; Wang Z; Klaunig JE; Glazier JA; PloS one 2018 Sep 13

A simple automated method for continuous fieldwise measurement of microvascular hemodynamics.
Clendenon SG; Fu X; Von Hoene RA; Clendenon JL; Sluka JP; Winfree S; Mang H; Martinez M; Filson AJ; Klaunig JE; Glazier JA; Dunn KW; Microvascular research 2018 Nov 28

Segmentation of biological images containing multitarget labeling using the jelly filling framework.
Gadgil NJ; Salama P; Dunn KW; Delp EJ; Journal of medical imaging (Bellingham, Wash.) 2018 Nov 23

Quantitative Large-Scale Three-Dimensional Imaging of Human Kidney Biopsies: A Bridge to Precision Medicine in Kidney Disease.
Winfree S; Dagher PC; Dunn KW; Eadon MT; Ferkowicz M; Barwinska D; Kelly KJ; Sutton TA; El-Achkar TM; Nephron 2018 Jun 5

Skeletal accumulation of fluorescently tagged zoledronate is higher in animals with early stage chronic kidney disease.
Swallow EA; Aref MW; Chen N; Byiringiro I; Hammond MA; McCarthy BP; Territo PR; Kamocka MM; Winfree S; Dunn KW; Moe SM; Allen MR; Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 2018 Jun 11

Kinetic Analysis of Vasculogenesis Quantifies Dynamics of Vasculogenesis and Angiogenesis In Vitro.
Varberg KM; Winfree S; Dunn KW; Haneline LS; Journal of visualized experiments : JoVE 2018 Jan 31

Live-Animal Imaging of Renal Function by Multiphoton Microscopy.
Dunn KW; Sutton TA; Sandoval RM; Current protocols in cytometry 2018 Jan 18

Intravital Multiphoton Microscopy with Fluorescent Bile Salts in Rats as an In Vivo Biomarker for Hepatobiliary Transport Inhibition.
Ryan J; Morgan RE; Chen Y; Volak LP; Dunn RT 2nd; Dunn KW; Drug metabolism and disposition: the biological fate of chemicals 2018 Feb 21

Imaging cell biology and physiology in vivo using intravital microscopy.
Dunn KW; Day RN; Methods (San Diego, Calif.) 2017 Sep 1

Two-Photon Intravital Fluorescence Lifetime Imaging of the Kidney Reveals Cell-Type Specific Metabolic Signatures.
Hato T; Winfree S; Day R; Sandoval RM; Molitoris BA; Yoder MC; Wiggins RC; Zheng Y; Dunn KW; Dagher PC; Journal of the American Society of Nephrology : JASN 2017 Mar 1

Large-scale 3-dimensional quantitative imaging of tissues: state-of-the-art and translational implications.
Winfree S; Ferkowicz MJ; Dagher PC; Kelly KJ; Eadon MT; Sutton TA; Markel TA; Yoder MC; Dunn KW; El-Achkar TM; Translational research : the journal of laboratory and clinical medicine 2017 Jul 22

Kinetic analyses of vasculogenesis inform mechanistic studies.
Varberg KM; Winfree S; Chu C; Tu W; Blue EK; Gohn CR; Dunn KW; Haneline LS; American journal of physiology. Cell physiology 2017 Jan 18

Quantitative Three-Dimensional Tissue Cytometry to Study Kidney Tissue and Resident Immune Cells.
Winfree S; Khan S; Micanovic R; Eadon MT; Kelly KJ; Sutton TA; Phillips CL; Dunn KW; El-Achkar TM; Journal of the American Society of Nephrology : JASN 2017 Feb 2

Using quantitative intravital multiphoton microscopy to dissect hepatic transport in rats.
Dunn KW; Ryan JC; Methods (San Diego, Calif.) 2017 Apr 21

A simple approach for measuring FRET in fluorescent biosensors using two-photon microscopy.
Day RN; Tao W; Dunn KW; Nature protocols 2016 Sep 29

Gene targets of mouse miR-709: regulation of distinct pools.
Surendran S; Jideonwo VN; Merchun C; Ahn M; Murray J; Ryan J; Dunn KW; Kota J; Morral N; Scientific reports 2016 Jan 8

Effects of chronic kidney disease on liver transport: quantitative intravital microscopy of fluorescein transport in the rat liver.
Ryan JC; Dunn KW; Decker BS; American journal of physiology. Regulatory, integrative and comparative physiology 2014 Oct 22

High resolution 4-dimension imaging of metanephric embryonic kidney morphogenesis.
Clendenon SG; Ward HH; Dunn KW; Bacallao R; Kidney international 2013 Jan 16

Live-animal imaging of renal function by multiphoton microscopy.
Dunn KW; Sutton TA; Sandoval RM; Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] 2012 Oct

Effects of IFN-? on intracellular trafficking and activity of macrophage NADPH oxidase flavocytochrome b558.
Casbon AJ; Long ME; Dunn KW; Allen LA; Dinauer MC; Journal of leukocyte biology 2012 Jul 20

Two-photon imaging within the murine thorax without respiratory and cardiac motion artifact.
Presson RG Jr; Brown MB; Fisher AJ; Sandoval RM; Dunn KW; Lorenz KS; Delp EJ; Salama P; Molitoris BA; Petrache I; The American journal of pathology 2011 May 7

Deep tissue fluorescent imaging in scattering specimens using confocal microscopy.
Clendenon SG; Young PA; Ferkowicz M; Phillips C; Dunn KW; Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada 2011 Jun 24

Endotoxin uptake by S1 proximal tubular segment causes oxidative stress in the downstream S2 segment.
Kalakeche R; Hato T; Rhodes G; Dunn KW; El-Achkar TM; Plotkin Z; Sandoval RM; Dagher PC; Journal of the American Society of Nephrology : JASN 2011 Jul 22

A practical guide to evaluating colocalization in biological microscopy.
Dunn KW; Kamocka MM; McDonald JH; American journal of physiology. Cell physiology 2011 Jan 5

The effects of refractive index heterogeneity within kidney tissue on multiphoton fluorescence excitation microscopy.
Young PA; Clendenon SG; Byars JM; Dunn KW; Journal of microscopy 2010 Sep 27

The effects of spherical aberration on multiphoton fluorescence excitation microscopy.
Young PA; Clendenon SG; Byars JM; Decca RS; Dunn KW; Journal of microscopy 2010 Oct 11

Rab10 associates with primary cilia and the exocyst complex in renal epithelial cells.
Babbey CM; Bacallao RL; Dunn KW; American journal of physiology. Renal physiology 2010 Jun 24

Improving Signal Levels in Intravital Multiphoton Microscopy using an Objective Correction Collar.
Muriello PA; Dunn KW; Optics communications 2008 Apr 1

Functional studies in living animals using multiphoton microscopy.
Dunn KW; Sutton TA; ILAR journal / National Research Council, Institute of Laboratory Animal Resources 2008

Live-animal imaging of renal function by multiphoton microscopy.
Dunn KW; Sutton TA; Sandoval RM; Current protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.] 2007 Jul

Chronic alcohol drinking alters neuronal dendritic spines in the brain reward center nucleus accumbens.
Zhou FC; Anthony B; Dunn KW; Lindquist WB; Xu ZC; Deng P; Brain research 2007 Jan 2

Principles of multiphoton microscopy.
Dunn KW; Young PA; Nephron. Experimental nephrology 2006 Mar 10

Rab10 regulates membrane transport through early endosomes of polarized Madin-Darby canine kidney cells.
Babbey CM; Ahktar N; Wang E; Chen CC; Grant BD; Dunn KW; Molecular biology of the cell 2006 Apr 26

Performance comparison between the high-speed Yokogawa spinning disc confocal system and single-point scanning confocal systems.
Wang E; Babbey CM; Dunn KW; Journal of microscopy 2005 May

Intra- and extravascular turnover of thyrotrophin-releasing hormone in normal man.
Iversen E; The Journal of endocrinology 1988 Sep