For the past thirty years I have dedicated my laboratory toward the study of acute kidney injury (AKI) from both ischemic and nephrotoxic compounds, primarily secondary to aminoglycosides.  We have helped to understand mechanistically, and developed therapeutically, many potential approaches for acute kidney injury. We have also spent the last ten years developing non-linear microscopy to quantify dynamic cellular and subcellular processes in vivo.  This has led to an NIH-P30 Imaging Core O’Brien Center of Excellence beginning in 2002 and recently renewed thru 2017.  The center involves MD’s, MD-PhD’s and PhD’s in a synergistic fashion.  Of particular importance is our advancement of fluorescent technologies from the bench to now the bedside.  Quantitative, mechanistic, therapeutic and toxicologic insights into disease processes have been produced.  We are now advancing our techniques and approaches to answer complex and novel therapeutic questions in humans with regard to GFR and plasma volume determinations, areas that have hindered the assessment, analysis and therapeutics in AKI and CKD.  We have also developed several unique rodent models allowing novel studies and mechanistic insights.