Mark C. Wagner, PHD

Faculty

Mark C. Wagner, PHD

Associate Research Professor of Medicine

Phone (317) 278-8784

Address

Research II, Suite E202
950 W Walnut St
R2 E202
Indianapolis, IN 46202-5188

Titles & Appointments

Associate Research Professor of Medicine
Associate Research Professor of Biochemistry & Molecular Biology

Education

1989 PhD Southwestern University

1989 PHD University of Texas Southwestern Medical Center

1989 PHD University of Texas Southwestern Medical Center

1983 BS Saint John'S University

1983 BS Saint John's University

1983 BS Saint John's University
Research

I am interested in determining the cellular function(s) myosin I b . Recent studies suggest that thisactin-based motor with both membrane and actin binding domains may contribute to a variety ofmembrane/actin processes such as endo/exocytosis, channel regulation and vesicle trafficking. I am usingkidney tubules as a model system to study this enzyme’s function and initial studies have been completedusing the LLCPK1 proximal tubule cell line (PTC). These cells contain an extensive actin rich brush border(BB) void of microtubules thus the dynamic membrane/cytoskeletal processes that occur in the BB willinvolve an actin motor. Using indirect immunofluorescence confocal microscopy I have identified myosin Ib in the BB of both LLCPK1 and rat kidney PTC. In addition, ATP depletion, in vitro, or ischemia, in vivo,causes dramatic alterations in this myosins distribution. I am currently analyzing the biochemical changesthat occur to myosin I b during this reversible pathophysiological process. These studies will utilize bothbiochemical and molecular approaches in addition to the microscopic analyses. Future studies are plannedto identify what myosin I b interacts with and how this potential motor is regulated. The techniques in useinclude, immunofluorescence, electron microscopy, microinjection, SDS-PAGE, chemiluminescence westernblotting, organelle isolations, immunoprecipitation, gene expression using adenovirus and GFP constructs,and antisense approaches to inhibit myosin I b .
Publications

Mechanism of increased clearance of glycated albumin by proximal tubule cells.
Wagner MC; Myslinski J; Pratap S; Flores B; Rhodes G; Campos-Bilderback SB; Sandoval RM; Kumar S; Patel M; Ashish; Molitoris BA; American journal of physiology. Renal physiology 2016 Feb 17

Proximal Tubules Have the Capacity to Regulate Uptake of Albumin.
Wagner MC; Campos-Bilderback SB; Chowdhury M; Flores B; Lai X; Myslinski J; Pandit S; Sandoval RM; Wean SE; Wei Y; Satlin LM; Wiggins RC; Witzmann FA; Molitoris BA; Journal of the American Society of Nephrology : JASN 2015 Jun 8

The proximal tubule and albuminuria: really!
Dickson LE; Wagner MC; Sandoval RM; Molitoris BA; Journal of the American Society of Nephrology : JASN 2014 Jan 9

Myo1c is an unconventional myosin required for zebrafish glomerular development.
Arif E; Kumari B; Wagner MC; Zhou W; Holzman LB; Nihalani D; Kidney international 2013 May 29

Multiple factors influence glomerular albumin permeability in rats.
Sandoval RM; Wagner MC; Patel M; Campos-Bilderback SB; Rhodes GJ; Wang E; Wean SE; Clendenon SS; Molitoris BA; Journal of the American Society of Nephrology : JASN 2012 Jan 5

Motor protein Myo1c is a podocyte protein that facilitates the transport of slit diaphragm protein Neph1 to the podocyte membrane.
Arif E; Wagner MC; Johnstone DB; Wong HN; George B; Pruthi PA; Lazzara MJ; Nihalani D; Molecular and cellular biology 2011 Mar 14

The antibiotic gentamicin inhibits specific protein trafficking functions of the Arf1/2 family of GTPases.
Lin L; Wagner MC; Cocklin R; Kuzma A; Harrington M; Molitoris BA; Goebl MG; Antimicrobial agents and chemotherapy 2010 Oct 18

Ischemic injury to kidney induces glomerular podocyte effacement and dissociation of slit diaphragm proteins Neph1 and ZO-1.
Wagner MC; Rhodes G; Wang E; Pruthi V; Arif E; Saleem MA; Wean SE; Garg P; Verma R; Holzman LB; Gattone V; Molitoris BA; Nihalani D; The Journal of biological chemistry 2008 Oct 14

CaMKII-mediated phosphorylation of the myosin motor Myo1c is required for insulin-stimulated GLUT4 translocation in adipocytes.
Yip MF; Ramm G; Larance M; Hoehn KL; Wagner MC; Guilhaus M; James DE; Cell metabolism 2008 Nov

A role for myosin 1e in cortical granule exocytosis in Xenopus oocytes.
Schietroma C; Yu HY; Wagner MC; Umbach JA; Bement WM; Gundersen CB; The Journal of biological chemistry 2007 Aug 16

Loss of the homotypic fusion and vacuole protein sorting or golgi-associated retrograde protein vesicle tethering complexes results in gentamicin sensitivity in the yeast Saccharomyces cerevisiae.
Wagner MC; Molnar EE; Molitoris BA; Goebl MG; Antimicrobial agents and chemotherapy 2006 Feb

Expression of the unconventional myosin Myo1c alters sodium transport in M1 collecting duct cells.
Wagner MC; Blazer-Yost BL; Boyd-White J; Srirangam A; Pennington J; Bennett S; American journal of physiology. Cell physiology 2005 Feb 16

Ischemia disrupts myosin I beta in renal tubules.
Boyd-White J; Srirangam A; Goheen MP; Wagner MC; American journal of physiology. Cell physiology 2001 Oct

Altered membrane-cytoskeleton linkage and membrane blebbing in energy-depleted renal proximal tubular cells.
Chen J; Wagner MC; American journal of physiology. Renal physiology 2001 Apr

Renal epithelial polarity in health and disease.
Wagner MC; Molitoris BA; Pediatric nephrology (Berlin, Germany) 1999 Feb

Ischemia activates actin depolymerizing factor: role in proximal tubule microvillar actin alterations.
Schwartz N; Hosford M; Sandoval RM; Wagner MC; Atkinson SJ; Bamburg J; Molitoris BA; The American journal of physiology 1999 Apr

ATP depletion alters myosin I beta cellular location in LLC-PK1 cells.
Wagner MC; Molitoris BA; The American journal of physiology 1997 May

Role of the actin cytoskeleton in ischemia-induced cell injury and repair.
Molitoris BA; Leiser J; Wagner MC; Pediatric nephrology (Berlin, Germany) 1997 Dec

Surface membrane polarity of proximal tubular cells: alterations as a basis for malfunction.
Molitoris BA; Wagner MC; Kidney international 1996 Jun

Identification of a 120 kd hair-bundle myosin located near stereociliary tips.
Gillespie PG; Wagner MC; Hudspeth AJ; Neuron 1993 Oct

Tissue distribution and subcellular localization of mammalian myosin I.
Wagner MC; Barylko B; Albanesi JP; The Journal of cell biology 1992 Oct

Purification and characterization of a mammalian myosin I.
Barylko B; Wagner MC; Reizes O; Albanesi JP; Proceedings of the National Academy of Sciences of the United States of America 1992 Jan 15

Purification of kinesin from bovine brain and assay of microtubule-stimulated ATPase activity.
Wagner MC; Pfister KK; Brady ST; Bloom GS; Methods in enzymology 1991

Modification of the microtubule-binding and ATPase activities of kinesin by N-ethylmaleimide (NEM) suggests a role for sulfhydryls in fast axonal transport.
Pfister KK; Wagner MC; Bloom GS; Brady ST; Biochemistry 1989 Nov 14

Submolecular domains of bovine brain kinesin identified by electron microscopy and monoclonal antibody decoration.
Hirokawa N; Pfister KK; Yorifuji H; Wagner MC; Brady ST; Bloom GS; Cell 1989 Mar 10

Monoclonal antibodies to kinesin heavy and light chains stain vesicle-like structures, but not microtubules, in cultured cells.
Pfister KK; Wagner MC; Stenoien DL; Brady ST; Bloom GS; The Journal of cell biology 1989 Apr

Copurification of kinesin polypeptides with microtubule-stimulated Mg-ATPase activity and kinetic analysis of enzymatic properties.
Wagner MC; Pfister KK; Bloom GS; Brady ST; Cell motility and the cytoskeleton 1989

Native structure and physical properties of bovine brain kinesin and identification of the ATP-binding subunit polypeptide.
Bloom GS; Wagner MC; Pfister KK; Brady ST; Biochemistry 1988 May 3

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