Research II, Suite E202
950 W Walnut St
Titles & Appointments
Associate Research Professor of Medicine
Associate Research Professor of Biochemistry & Molecular Biology
1989PhDUniversity of Texas Southwestern Medical Center
1983BSSaint John's University
I am interested in determining the cellular function(s) myosin I b . Recent studies suggest that thisactin-based motor with both membrane and actin binding domains may contribute to a variety ofmembrane/actin processes such as endo/exocytosis, channel regulation and vesicle trafficking. I am usingkidney tubules as a model system to study this enzyme’s function and initial studies have been completedusing the LLCPK1 proximal tubule cell line (PTC). These cells contain an extensive actin rich brush border(BB) void of microtubules thus the dynamic membrane/cytoskeletal processes that occur in the BB willinvolve an actin motor. Using indirect immunofluorescence confocal microscopy I have identified myosin Ib in the BB of both LLCPK1 and rat kidney PTC. In addition, ATP depletion, in vitro, or ischemia, in vivo,causes dramatic alterations in this myosins distribution. I am currently analyzing the biochemical changesthat occur to myosin I b during this reversible pathophysiological process. These studies will utilize bothbiochemical and molecular approaches in addition to the microscopic analyses. Future studies are plannedto identify what myosin I b interacts with and how this potential motor is regulated. The techniques in useinclude, immunofluorescence, electron microscopy, microinjection, SDS-PAGE, chemiluminescence westernblotting, organelle isolations, immunoprecipitation, gene expression using adenovirus and GFP constructs,and antisense approaches to inhibit myosin I b .
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