48163-Imanishi, Yoshikazu
Faculty

Yoshikazu Imanishi, PhD

Associate Professor of Ophthalmology

Address
GK 400A
OPTH
IN
Indianapolis, IN

Bio

PROFESSIONAL APPOINTMENTS

Aug 2020 – date                    Primary member, Stark Neurosciences Research Institute, Indiana University School of Medicine, Indianapolis, Indiana.

July 2020 – date                    Adjunct faculty member, Department of Pharmacology and Toxicology, Indiana University School of Medicine, Indianapolis, Indiana.

July 2020 – date                    Associate Professor (with tenure), Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana.

July 2020 – date                    Director of Research Mentoring for the Department of Ophthalmology, Indiana University School of Medicine, Indianapolis, Indiana.

July 2020 – date                    Lois Letzter Chair in Ophthalmology

July 2017 – July 2020           Associate Professor (with tenure), Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio.

Dec., 2009 – June 2017        Assistant Professor (tenure track), Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio.

Jan,, 2006 – Nov., 2009        Assistant Professor (non-tenure track), Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio. 

Sept., 2005 – Dec., 2005,     Research Associate, Department of Pharmacology, Case Western Reserve University, Cleveland, Ohio.  Laboratory of Krzysztof Palczewski, Ph.D. 

Apr., 2000 – Aug., 2005,       Senior Fellow, Department of Ophthalmology, University of Washington, Seattle.  Laboratory of Krzysztof Palczewski, Ph.D.  

Key Publications

RPE cells engulf microvesicles secreted by degenerating rod photoreceptors. Ropelewski P and Imanishi Y eNeuro 6 May 2020, ENEURO.0507-19.2020; DOI: https://doi.org/10.1523/ENEURO.0507-19.2020

Disrupted plasma membrane protein homeostasis in a Xenopus laevis model of retinitis pigmentosa. Ropelewski P and Imanishi Y J Neurosci. 2019 Jul 10;39(28):5581-5593. doi: 10.1523/JNEUROSCI.3025-18.2019.

Müller glia phagocytose dead photoreceptor cells in a mouse model of retinal degenerative disease. Sakami S, Imanishi Y*, Palczewski K*. The FASEB Journal. FASEB J. 2019 Mar;33(3):3680-3692. doi: 10.1096/fj.201801662R. (* corresponding authors)

A small molecule mitigates hearing loss in a mouse model of Usher syndrome III. Alagramam KN*, Gopal SR, Geng R, Chen DH, Nemet I, Lee R, Tian G, Miyagi M, Malagu KF, Lock CJ, Esmieu WR, Owens AP, Lindsay NA, Ouwehand K, Albertus F, Fischer DF, Bürli RW, MacLeod AM, Harte WE, Palczewski K, Imanishi Y*. Nat Chem Biol. 2016 Apr 25. doi: 10.1038/nchembio.2069. (* corresponding authors) (Featured by “News and Views” of the journal)

 

Impairment of vision in a mouse model of Usher Syndrome type III.  Tian G, Lee R, Ropelewski P, Imanishi Y. Invest Ophthalmol Vis Sci. 2016 Mar 1;57(3):866-75. doi: 10.1167/iovs.15-16946.

Organization of cGMP sensing structures on the rod photoreceptor outer segment plasma membrane.  Nemet I, Tian G, Imanishi Y. Channels (Austin). 2014;8(6):528-35. PubMed PMID: 25616687. (Invited research article, Featured by “News and Views” of the journal)

Retrograde intraciliary trafficking of opsin during the maintenance of cone-shaped photoreceptor outer segments of Xenopus laevis.  Tian G, Lodowski KH, Lee R, Imanishi Y. J Comp Neurol. 2014 Nov 1;522(16):3577-89. Featured on the cover

Submembrane Assembly and Renewal of Rod Photoreceptor cGMP-Gated Channel: Insight into the Actin-Dependent Process of Outer Segment Morphogenesis.  Nemet I, Tian G, Imanishi Y. J Neurosci. 2014 Jun 11;34(24):8164-74. Selected as a Featured Article in the Journal of Neuroscience

An unconventional secretory pathway mediates the cilia targeting of peripherin/rds.  Tian G, Ropelewski P, Nemet I, Lee R, Lodowski KH, and Imanishi Y. J. Neurosci. 2014 Jan 15;34 992-1006  Recommended by F1000Prime

Signals governing the trafficking and mis-trafficking of a ciliary GPCR, rhodopsin.  Lodowski KH, Lee R, Ropelewski P, Nemet I, Tian G, Imanishi Y. J Neurosci. 2013 Aug 21;33(34):13621-38.

The mechanosensory structure of the hair cell requires clarin-1, a protein encoded by Usher syndrome III causative gene.  Geng R, Melki S, Chen DH, Tian G, Furness DN, Oshima-Takago T, Neef J, Moser T, Askew C, Horwitz G, Holt JR, Imanishi Y, Alagramam KN. J Neurosci. 2012 Jul 11;32(28):9485-98.

Noninvasive multiphoton fluorescence microscopy resolves retinol and retinal condensation products in mouse eyes.  Palczewska G, Maeda T, Imanishi Y, Sun W, Chen Y, Williams DR, Piston DW, Maeda A, Palczewski K.  Nat Med. 2010 Dec;16(12):1444-9.

Clarin-1, encoded by the Usher syndrome III causative gene, forms a membranous microdomain: Possible role of Clarin-1 in organizing the actin cytoskeleton.  Tian G, Zhou Y, Hajkova D, Miyagi M, Dinculescu A, Hauswirth WW, Palczewski K, Geng R, Alagramam KN, Isosomppi J, Sankila EM, Flannery JG, Imanishi Y.  J Biol Chem. 2009 May 7.  doi:10.1074/jbc.M109.003160

Retinyl ester homeostasis in the adipose differentiation-related protein-deficient retina.  Imanishi Y*, Sun W, Maeda T, Maeda A, Palczewski K*.  J Biol Chem. 2008 Sep 5;283(36):25091-102. (* corresponding authors)

RBP4 disrupts vitamin A uptake homeostasis in a STRA6-deficient animal model for Matthew-Wood syndrome.  Isken A, Golczak M, Oberhauser V, Hunzelmann S, Driever W, Imanishi Y, Palczewski K, von Lintig J.  Cell Metab. 2008 Mar;7(3):258-68. Featured on the cover

Essential role of Ca2+-binding protein 4, a Cav1.4 channel regulator, for photoreceptor synaptic function.  Haeseleer F*, Imanishi Y*, Maeda T, Possin DE, Maeda A, Lee A, Rieke F, and Palczewski K.  Nature Neuroscience. 2004; Oct;7(10):1079-87. (*  authors contributed equally to the work) Recommended by F1000Prime

Diversity of guanylate cyclase-activating proteins (GCAPs) in teleost fish: Characterization of three novel GCAPs (GCAP4, GCAP5, GCAP7 ) from zebrafish (Danio Rerio) and prediction of eight GCAPs (GCAP1-8) in pufferfish (Fugu Rubripes).  Imanishi Y*, Yang L*, Sokal I, Filipek S, Palczewski K, Baehr W.  J Mol Evol. 2004; 59: 204-217 (*  authors contributed equally to the work)

Lecithin:retinol acyltransferase (LRAT) is essential for retention of retinyl esters in the eye and in the liver.  Batten ML*, Imanishi Y*, Maeda T*, Tu D, Moise AR, Bronson D, Possin D, Van Gelder RN, Baehr W, Palczewski K.  J Biol Chem. 2004 Mar 12;279(11):10422-32. (*  authors contributed equally to the work)

Noninvasive two-photon imaging reveals retinyl ester storage structures in the eye.  Imanishi Y, Batten ML, Piston DW, Baehr W, Palczewski K.  J Cell Biol. 2004 Feb 2;164(3):373-83. Featured on the cover and by “In This Issue of the journal

Dual-substrate specificity short chain retinol dehydrogenases from the vertebrate retina.  Haeseleer F*, Jang GF*, Imanishi Y*, Driessen CA, Matsumura M, Nelson P, Palczewski K.  J Biol Chem. 2002 Nov 22;277(47):45537-46. (*  authors contributed equally to the work)

Characterization of retinal guanylate cyclase-activating protein 3 (GCAP3) from zebrafish to man.  Imanishi Y, Li N, Sokal I, Sowa ME, Lichtarge O, Wensel TG, Saperstein DA, Baehr W, Palczewski K.  Eur. J. Neurosci. 2002;15(1):63-78. Featured on the cover

Titles & Appointments

  • Associate Professor of Ophthalmology
  • Letzter Scholar in Ophthalmology
  • Education
    2000 PhD Osaka University
    1997 MSC Osaka University
    1995 BSC Osaka University
  • Research

    (a)     Morphogenesis and maintenance of membrane structures in photoreceptor neurons

    (b)     Ciliary protein trafficking and ciliopathy in the retina

    (c)     Invention of technologies for better understanding of cell biology

  • Professional Organizations
    Association for Research in Vision and Ophthalmology
    International Society for Eye Research
    Society for Neuroscience
  • Clinical Interests

    (a)     Drug discovery for inherited blinding disorders

    (b)     Development of new models emulating human blinding disorders

  • Awards
    Org: Indiana University
    Desc: Lois Letzter Chair in Ophthalmology
    Scope: University
    Date: 2020-07-15
    Org: Lighthouse Guild
    Desc: Pisart Award in Vision Research
    Scope: International
    Date: 2017-10-20
    Org: Harvard Medical School, Department of Ophthalmology, Boston Children’s Hospital
    Desc: Visiting Professor Lecture Series
    Scope: National
    Date: 2017-09-27

Looking for patient care?

To schedule an appointment with a faculty member physician of IU School of Medicine, contact Indiana University Health at 888-484-3258 or use the physician finder by clicking the button below.

Find a doctor