43064-Singh, Kanhaiya
Faculty

Kanhaiya Singh, PhD

Assistant Professor of Surgery

Email kanh@iu.edu
Phone (317) 278-3411
Address
975 W Walnut St.
IB 444

Indianapolis, IN 46202

Bio

Dr. Singh currently serves as Assistant Professor of Surgery, Division of Plastic Surgery, Department of Surgery at Indiana University. Dr. Singh received his doctoral degree in Biotechnology from Banaras Hindu University, India. Prior to joining Indiana University, Dr. Singh completed his post-doctoral research at The Ohio State University. Dr. Singh’s research focus is on epigenetic mechanisms of gene expression during diabetic vasculopathy and includes single-cell transcriptomics and single-cell ATAC sequencing approaches to investigate the molecular pathways that are compromised. Dr. Singh served as contributor to more than 40 peer reviewed publications.

Key Publications

Singh K*, Rustagi Y, Abouhashem AS, Tabasum S, Verma P, Hernandez E, Pal D, Khona DK, Mohanty SK, Kumar M, Srivastava R, Guda PR, Verma SS, Mahajan S, Killian JA, Walker LA, Ghatak S, Mathew-Steiner SS, Wanczyk K, Liu S, Wan J, Yan P, Bundschuh R, Khanna S, Gordillo GM, Murphy MP, Roy S and Sen CK*. Genome-wide DNA hypermethylation opposes healing in chronic wound patients by impairing epithelial-to-mesenchymal transition. J Clin Invest. 2022;132(17):e157279. (*co-corresponding authors).

Rustagi Y, Abouhashem AS, Verma P, Verma SS, Hernandez E, Liu S, Kumar M, Guda PR, Srivastava R, Mohanty SK, Kacar S, Mahajan S, Wanczyk KE, Khanna S, Murphy MP, Gordillo GM, Roy S, Wan J, Sen CK, Singh K. Endothelial Phospholipase Cγ2 Improves Outcomes of Diabetic Ischemic Limb Rescue Following VEGF Therapy. Diabetes. 2022 May 1;71(5):1149-1165.

Singh K, Sinha M, Pal D, Tabasum S, Gnyawali SC, Kona D, Sarkar S, Mohanty SK, Soto-Gonzalez F, Khanna S, Roy S, Sen CK. Cutaneous epithelial to mesenchymal transition activator ZEB1 regulates wound angiogenesis and closure in a glycemic status dependent manner. Diabetes 2019, Nov;68(11):2175-2190.

Singh K, Pal D, Sinha M, Ghatak S, Gyawali S, Khanna S, Roy S, Sen CK. (2017) Epigenetic modification of microRNA-200b contributes to diabetic vasculopathy. Molecular Therapy. Molecular Therapy. 25 (12), 2689-2704.

Sinha M, Sen CK, Singh K, Das A, Ghatak S, Rhea B, Blackstone B, Powell H, Khanna S, Roy S. Direct conversion of injury-site myeloid cells to fibroblast-like cells of granulation tissue. Nature Communications 2018; 9 (1): 936. 

Gallego-Perez D, Pal D, Ghatak S, Malkoc V, Higuita-Castro N, Gnyawali S, Chang L, Liao WC, Shi J, Sinha M, Singh K, Steen E, Sunyecz A, Stewart R, Moore J, Ziebro T, Northcutt RG, Homsy M, Bertani P, Lu W, Roy S, Khanna S, Rink C, Sundaresan VB, Otero JJ, Lee LJ and Sen CK. Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue. Nat Nanotechnol. 2017;12:974-979.

Titles & Appointments

  • Assistant Professor of Surgery
  • Education
    2015 PhD Banaras Hindu University
    2010 BTECH Sam Higginbottom University of Agriculture, Technology and Sciences
  • Research
    Dr. Singh’s laboratory at the Indiana Center for Regenerative Medicine and Engineering (ICRME) focuses on epigenetics of diabetic vasculopathy and wound healing.

    Wound healing: His laboratory is investigating the epigenetic mechanisms such as DNA methylation as a proxy for abnormal gene–environmental interactions in diabetic ischemic wounds. Current line of study aims to develop a simple and point of care approach to achieve targeted epigenetic editing of adult diabetic ischemic tissue in vivo. The research is funded through peer-reviewed grant from Department of Defense (DoD).

    Diabetic Vasculopathy: His laboratory is also investigating the molecular mechanism in diabetic vasculopathy and limb ischemia. His laboratory is applying single-cell RNA sequencing technology to study the endothelial cells of the human diabetic skin to identify novel ligands and receptors that can improve outcomes of the VEGF therapy. To that end, his team is employing ICRME’s Tissue Nanotransfection technology as a delivery platform to administer combination gene therapy for diabetic ischemic limb rescue.   

    Active Grants

    DoD W81XWH-22-1-0146 (PI)

    DoD W81XWH-21-1-0033 (Co-I)

    NIH R01DK128845 (Co-I)

    John Templeton Foundation, 61742 (Co-I)

    Google Scholar 

  • Professional Organizations
    American Diabetes Association
    Wound Healing Society

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