Research in my laboratory applies quantitative fluorescence microscopy techniques to visualize the behaviors of proteins inside living cells. Our studies use the combination of molecular biology, biochemistry, and live-cell imaging approaches to determine how specific signal transduction and gene regulatory complexes are assembled. We are using time-resolved fluorescence lifetime imaging microscopy (FLIM) to measure Förster resonance energy transfer (FRET), which enables us to determine how certain disease-causing point mutations can affect the assembly of specific protein complexes. I have over one hundred publications, with extensive experience in live-cell imaging. In addition, I have taught in the annual Cold Spring Harbor Laboratory or Marine Biological Laboratory live-cell imaging courses yearly since 1998, and I am currently the co-organizer for the Workshop on FRET microscopy, held annually at the University of Virginia since 2001.