17979-Obukhov, Alexander

Alexander G. Obukhov, PhD

Associate Professor of Anatomy, Cell Biology & Physiology

Email
aobukhov@iu.edu
Phone
317-274-8078
Address
635 Barnhill Dr., MS 360A

Indianapolis, IN 46202
PubMed:

Bio

Alexander Obukhov studied cellular electrophysiology and neurophysiology in the Bogomoletz Institute of Physiology at the Ukrainian National Academy of Sciences (Kiev, Ukraine) in the laboratory of Dr. Oleg Krishtal and earned his PhD degree in 1988. His early research focused on determining the single-channel properties and pharmacology of ATP-activated cation channels (now known as P2x receptor-channels) in dorsal root ganglion and nodosum sensory neurons. During his first postdoctoral training in the laboratory of Dr. Oleg Krishtal, Dr. Obukhov studied the pharmacological properties of voltage-gated calcium channels in hippocampal and Purkinje neurons. Dr. Obukhov received his second postdoctoral training in the laboratory of Dr. Günter Schultz in the Institute of Pharmacology at the Benjamin Franklin University Clinic of Freie Universität Berlin (West Berlin, Germany). He investigated the mechanisms of regulation and electrophysiological and pharmacological properties of newly cloned TRP channels. Dr. Obukhov continued his studies of TRP channels when he moved to the US in 1999 and took a faculty position in the Department of Physiology and Pharmacology at the University of Medicine and Dentistry of New Jersey. Dr. Obukhov joined the Indiana University School of Medicine faculty in 2006. His laboratory currently focuses on establishing the role of TRPC channels in the metabolic syndrome-associated atherosclerosis using the Ossabaw pig model. The other projects investigate the mechanisms regulating the contractility of the uterus, the mechanisms underlying endothelial dysfunction in a mouse aorta model, traumatic brain injury-associated vascular dysfunction, diabetic neuropathy, catecholamine secretion regulation in adrenal chromaffin cells, and the function of SLC4A11 transporters in the corneal endothelium. Dr. Obukhov serves on study sections for the American Heart Association and on the Editorial Boards of Scientific Reports, Cells, and PLOS One. Dr. Obukhov is a member of the American Heart Association and the Society for Neuroscience.

 

Honors and Awards
1993 - Awarded an Alexander von Humboldt Research Fellowship to conduct Postdoctoral Research in Germany
1996 - Received two Research Awards from Benjamin Franklin University Clinic, Freie Universität Berlin (West Berlin, Germany) for studies of TRPL and TRPC1 channels -
Obukhov AG, Harteneck C, Zobel A, Harhammer R, Kalkbrenner F, Leopoldt D, Lückhoff A, Nürenberg B, Schultz G. "Activation of TRPL cation channels by Galpha11 subinits." 
Zitt C, Zobel A, Obukhov AG, Harteneck C, Kalkbrener F, Lückhoff A, Schultz G. "Cloning and characterization of human TRPC1."
1999 - Received a Research Award from Benjamin Franklin University Clinic, Freie Universität Berlin (West Berlin, Germany) for studies on TRPC6 and TRPC3 channels -
Hofmann T, Obukhov AG, Schaefer M, Gudermann T, Schultz G. "Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol."
2009 Received two Research Awards at the CTSI-IU School of Medicine Poster competition-Kumar S, Barbosa C, Chakraborty S, Obukhov AG “The role of TRPC channels in regulating neurite length.”
Neeb Z., Obukhov AG, Long X, Sturek M “Store-operated Ca2+ influx in coronary smooth muscle cells predicts coronary atherosclerosis in the metabolic syndrome.”
2010 - American Heart Association Research Administration Volunteer Recognition Award
2012 - Trustee's Teaching Award from the Indiana University School of Medicine  
2016 - American Heart Association Research Administration Volunteer Recognition Award

 
Teaching
Lectured in:
Biomedical Science I. Biochemical Basis of Biological Processes (IN-GRDM-G715)
Cardiovascular, Renal and Respiratory Function in Health and Disease (IN-GRDM-G735)
Gastrointestinal and Endocrine Function in Health and Disease (IN-GRDM-G736)
Foundation of Electrical Signaling and Ion Channels (IN-GRDM-G743)
Foundation of Neuroscience (IN-GRDM-G780)
Molecular Physiology of Ion Transport (IN-GRDM-G761)
Foundation of Clinical Sciences (IN-ANES-B512)
Fundamentals of Health and Disease (IN-IUSM-93XM640)
Human Physiology (IN-PHSL-F503)
Clinical Physiology & Pathophysiology I for Physician Assistants (IN-MPAS-M531)
Clinical Physiology & Pathophysiology II for Physician Assistants (IN-MPAS-532)

Facilitator in:
Endocrine & Reproductive Biology (IN-IUSM-93XM760)
Gastrointestinal and Nutrition (IN-IUSM-93XM740)

Course Director of:
Molecular Physiology of Ion Transport (IN-GRDM-G761)

Key Publications

Chen X, Duan Y, Riley AM, Welch MA, White FA, Grant MB, Obukhov AG. Long-Term Diabetic Microenvironment Augments the Decay Rate of Capsaicin-Induced Currents in Mouse Dorsal Root Ganglion Neurons. Molecules. 2019; 24(4): 775. doi: 10.3390/molecules24040775.

Chen X, Taylor-Nguyen NN, Riley AM, Herring BP, White FA, Obukhov AG. The TRPC6 inhibitor, larixyl acetate, is effective in protecting against traumatic brain injury-induced systemic endothelial dysfunction. J Neuroinflammation. 2019; 16(1): 21. doi: 10.1186/s12974-019-1407-6.

Ma W, Chen X, Cerne R, Syed SK, Ficorilli JV, Cabrera O, Obukhov AG, Efanov AM. Catechol estrogens stimulate insulin secretion in pancreatic β-cells via activation of the transient receptor potential A1 (TRPA1) channel. J Biol Chem. 2019; 294(8): 2935-2946. doi: 10.1074/jbc.RA118.005504. 

Li S, Hundal KS, Chen X, Choi M, Ogando DG, Obukhov AG, Bonanno JA. R125H, W240S, C386R, and V507I SLC4A11 mutations associated with corneal endothelial dystrophy affect the transporter function but not trafficking in PS120 cells. Exp Eye Res. 2019; 180:86-91. doi: 10.1016/j.exer.2018.12.003. 

Chen X, Liu D, Zhou D, Si Y, Xu D, Stamatkin CW, Ghozayel MK, Ripsch MS, Obukhov AG, White FA, Meroueh SO. Small-molecule CaVα1⋅CaVβ antagonist suppresses neuronal voltage-gated calcium-channel trafficking. Proc Natl Acad Sci U S A. 2018; 115(45): E10566-E10575. doi: 10.1073/pnas.1813157115. 

Chen X, Meroueh M, Mazur G, Rouse E, Hundal KS, Stamatkin CW, Obukhov AG. Phenylephrine, a common cold remedy active ingredient, suppresses uterine contractions through cAMP signalling. Sci Rep. 2018; 8(1): 11666. doi: 10.1038/s41598-018-30094-5.

Li W, Chen X, Riley AM, Hiett SC, Temm CJ, Beli E, Long X, Chakraborty S, Alloosh M, White FA, Grant MB, Sturek M, Obukhov AG. Long-term spironolactone treatment reduces coronary TRPC expression, vasoconstriction, and atherosclerosis in metabolic syndrome pigs. Basic Res Cardiol. 2017; 112(5): 54.

Chen X, Li W, Riley AM, Soliman M, Chakraborty S, Stamatkin CW, Obukhov AG. Molecular Determinants of the Sensitivity to Gq/11-Phospholipase C-dependent Gating, Gd3+ Potentiation, and Ca2+ Permeability in the Transient Receptor Potential Canonical Type 5 (TRPC5) Channel. J Biol Chem. 2017; 292(3): 898-911.

Chen X, Li W, Hiett SC, Obukhov AG. Novel Roles for Kv7 Channels in Shaping Histamine-Induced Contractions and Bradykinin-Dependent Relaxations in Pig Coronary Arteries. PLoS One. 2016; 11(2):e0148569.

Zhang W, Ogando DG, Bonanno JA, Obukhov AG. Human SLC4A11 Is a Novel NH3/H+ Co-transporter. J Biol Chem. 2015; 290(27): 16894-16905.

Chen X, Sun W, Gianaris NG, Riley AM, Cummins TR, Fehrenbacher JC, Obukhov AG. Furanocoumarins are a novel class of modulators for the transient receptor potential vanilloid type 1 (TRPV1) channel. J Biol Chem. 2014; 289(14): 9600-9610.

Chakraborty S, Berwick ZC, Bartlett PJ, Kumar S, Thomas AP, Sturek M, Tune JD, Obukhov AG. Bromoenol lactone inhibits voltage-gated Ca2+ and transient receptor potential canonical channels. J Pharmacol Exp Ther. 2011; 339(2): 329-340.

Kumar S, Chakraborty S, Barbosa C, Brustovetsky T, Brustovetsky N, Obukhov AG. Mechanisms controlling neurite outgrowth in a pheochromocytoma cell line: the role of TRPC channels. J Cell Physiol. 2012; 227(4): 1408-1419.

Edwards JM, Neeb ZP, Alloosh MA, Long X, Bratz IN, Peller CR, Byrd JP, Kumar S, Obukhov AG, Sturek M. Exercise training decreases store-operated Ca2+ entry associated with metabolic syndrome and coronary atherosclerosis. Cardiovasc Res. 2010; 85(3): 631-640.

Hu G, Oboukhova EA, Kumar S, Sturek M, Obukhov AG. Canonical transient receptor potential channels expression is elevated in a porcine model of metabolic syndrome. Mol Endocrinol. 2009; 23(5): 689-699.

Obukhov AG, Nowycky MC. A cytosolic residue mediates Mg2+ block and regulates inward current amplitude of a transient receptor potential channel. J Neurosci. 2005; 25(5):1234-1239.

Obukhov AG, Nowycky MC. TRPC5 activation kinetics are modulated by the scaffolding protein ezrin/radixin/moesin-binding phosphoprotein-50 (EBP50). J Cell Physiol. 2004; 201(2): 227-235.

Obukhov AG, Nowycky MC. TRPC4 can be activated by G-protein-coupled receptors and provides sufficient Ca2+ to trigger exocytosis in neuroendocrine cells. J Biol Chem. 2002; 277(18): 16172-16178.

Hofmann T, Obukhov AG, Schaefer M, Harteneck C, Gudermann T, Schultz G. Direct activation of human TRPC6 and TRPC3 channels by diacylglycerol. Nature. 1999; 397(6716): 259-263.

Obukhov AG, Harteneck C, Zobel A, Harhammer R, Kalkbrenner F, Leopoldt D, Lückhoff A, Nürnberg B, Schultz G. Direct activation of trpl cation channels by Galpha11 subunits. EMBO J. 1996; 15(21): 5833-5838.

Krishtal OA, Marchenko SM, Obukhov AG. Cationic channels activated by extracellular ATP in rat sensory neurons. Neuroscience. 1988; 27(3): 995-1000.

 

Research Labs

Faculty research at IU School of Medicine is transforming health. Details about the medical research being conducted in faculty labs throughout IU School of Medicine are available in the Research section of this site.
Faculty Labs