Pratibha Singh, PhD
Assistant Research Professor of Medicine
Pratibha Singh, Yongxue Yao, Abigail Weliver, Hal E. Broxmeyer, Soon-Cheol Hong, Cheong-Hee Chang., Vaccinia virus infection modulates the hematopoietic cell compartments in the bone marrow. Stem Cells. 2008 Apr; 26(4):1009-16.
Jonathan Hoggatt, Pratibha Singh, Janardhan Sampath, and Louis M. Pelus, Prostaglandin E2enhances hematopoietic stem cell homing, survival, and proliferation. Blood. 2009 May 28; 113 (22):5444-55.
Pratibha Singh, Jonathan Hoggatt, Peirong Hu, Jennifer M. Speth, Seiji Fukuda, Richard M. Breyer, and Louis M. Pelus, Blockade of prostaglandin E2signaling through EP1 and EP3 receptors attenuates Flt3L-dependent dendritic cell development from hematopoietic progenitor cells. Blood. 2012 Feb 16; 119(7):1671-1682.
Pratibha Singh, Peirong Hu, Jonathan Hoggatt, Akira Moh and Louis M. Pelus,
Expansion of bone marrow neutrophils following G-CSF administration in mice results in osteolineage cell apoptosis and mobilization of hematopoietic stem and progenitor cells.
Leukemia. 2012 Nov;26(11):2375-83.
Jonathan Hoggatt, Khalid S Mohammad†,Pratibha Singh†, Hoggatt AF, Chitteti BR, Speth JM, Hu P, Poteat BA, Stilger KN, Ferraro F, Silberstein L, Wong FK, Farag SS, Czader M, Milne GL, Breyer RM, Serezani CH, Scadden DT, Guise TA, Srour EF, Pelus LM., Differential stem- and Progenitor-cell trafficking by prostaglandin E2.Nature. 2013 Mar 21;495(7441):365-9. (†contributed equally).
Pratibha Singh, Louis M. Pelus. IFN-1 Bid crosstalk: foe or friend to stem cells. Stem Cell Investig. 2017 Feb 27; 4:18. doi: 10.21037/sci.2017.02.06. eCollection 2017.
Pratibha Singh, Jonathan Hoggatt, Malgorzata M. Kamocka, Khalid S. Mohammad, Mary R. Saunders, Hongge Li, Jennifer Speth, Nadia Carlesso, Theresa A Guise and Louis M. Pelus. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells. J Clin Invest.2017;127(12):4527-4540.
Jonathan Hoggatt, Pratibha Singh, Tiffany A. Tate, Bin-Kaun Chou, Shruti R. Datari, Seiji Fukuda, Liqiong Liu, Peter V. Kharchenko, Amir Schajnovitz, Ninib Baryawno, Francois E. Mercier, Joseph Boyer, Jason Gardner, Dwight M. Marrow, David T. Scadden. Rapid Mobilization Reveals a Highly Engraftable Hematopoietic Stem Cell. Cell. 2018 Jan 11;172(1-2):191-204.
Pratibha Singh, Seiji Fukuda, Liqiong Liu, Brahmananda Reddy Chitteti, Louis M. Pelus. Survivin Is Required for Mouse and Human Bone Marrow Mesenchymal Stromal Cell Function. Stem Cells. 2018 Jan;36(1):123-129.
My research focuses on understanding mechanisms that regulate normal and stressed hematopoietic stem and progenitor proliferation, differentiation and survival, and the role played by bone marrow niche support cells in hematopoietic stem cell function.
Current clinical hematopoietic transplant (HCT) relies on moving HSPC from bone marrow (BM) to the circulation, a process called peripheral blood stem cell mobilization (PBSCM), with G-CSF (Neupogen) being the primary drug used clinically for PBSCM. However, little is known about the mechanisms governing HSPC egress. Understanding this process has major relevance for clinical HCT. Poor homing and inadequate expansion, survival and differentiation of transplanted HSPC can limit transplant efficacy. Understansing hematopoietic stem cell regulation will allow us to develop improved stratiegies for hematopoietic stem cell based therapies, and facilitate recovery of hematopoieisis following stress and injury such as chemo/radio therapy or acute radiation exposure. My research interests include identifying mechanisms of action of Neuropeptide Y on hematopoietic cells and on the function of stromal niche support cells and in regulation of hematopoietic stem and progenitor cells trafficking. I am currently exploring the role of sinusoidal endothelial cells in hematopoietic stem cell maintenance and trafficking and have identified an unappreciated role for DPP IV- Neuropeptide Y axis in regulation of endothelial cell barrier permeability and hematopoietic stem cell trafficking. I am also investigating the role of Survivin in regulating mesenchymal stem cell functions and I have identified a previously unknown role for Survivin in the mesenchymal stem cell survival/proliferation and maintenance of hematopoietic stem cell niche. My emerging interest is to investigate the role of stromal cell populations in regulation of hematopoiesis following stress, particularly in niche regeneration after myeloablative injury such as radiation exposure.