Corson Lab protocols range from the routine to the esoteric best practices and tips. External research labs are invited to both use and share these protocols, with appropriate attribution to the Corson Lab at IU School of Medicine.
Guidelines and general procedures are offered for culturing, subculturing and freezing adherent cells within a traditional lab setting.
In order to avoid culture contamination for optimal results, follow these best practices.
Explore handy recipes for bis-tris polyacrylamide gels designed for optimal separation of a wide range of proteins.
Determine characteristics of protein antigens within complex mixtures using Laemmli and bis-tris protocols.
Confirm cellular anchorage-independent growth using the soft agar colony assay method.
Consider an alternative method to whole-cell pulldown that involves cell fractionation using biotinylated affinity reagents.
Best practices are outlined to achieve optimal results for mass spectrometry compatible silver staining.
Due to its liable nature within the lab, simple precautions will reduce the degradation of RNA samples.
Simple instructions are provided to enhance high-resolution graphic files for PowerPoint presentations.