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Faculty Research Labs Faculty Research Labs

Bis-Tris Gel Recipes

1 gel: 8% 10% 12% 15% Stacking
3.5x buffer, mL 1.42 1.42 1.42 1.42 0.5
30% acrylamide, mL 1.34 1.67 2 2.5 0.23
H2O, mL 2.24 1.91 1.58 1.08 1.02
10% APS, µL 25 25 25 25 20
TEMED, µL 7 7 7 7 10
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

2 gels: 8% 10% 12% 15% Stacking
3.5x buffer, mL 2.84 2.84 2.84 2.84 1
30% acrylamide, mL 2.68 3.34 4 5 0.46
H2O, mL 4.48 3.82 3.16 2.16 2.04
10% APS, µL 50 50 50 50 40
TEMED, µL 14 14 14 14 20
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

3 gels: 8% 10% 12% 15% Stacking
3.5x buffer, mL 4.26 4.26 4.26 4.26 1.5
30% acrylamide, mL 4.02 5.01 6 7.5 0.69
H2O, mL 6.72 5.73 4.74 3.24 3.06
10% APS, µL 75 75 75 75 60
TEMED, µL 21 21 21 21 30
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

4 gels: 8% 10% 12% 15% Stacking
3.5x buffer, mL 5.68 5.68 5.68 5.68 2
30% acrylamide, mL 5.36 6.68 8 10 0.92
H2O, mL 8.96 7.64 6.32 4.32 4.08
10% APS, µL 100 100 100 100 80
TEMED, µL 28 28 28 28 40
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl