1 gel: | 8% | 10% | 12% | 15% | Stacking |
---|---|---|---|---|---|
3.5x buffer, mL | 1.42 | 1.42 | 1.42 | 1.42 | 0.5 |
30% acrylamide, mL | 1.34 | 1.67 | 2 | 2.5 | 0.23 |
H2O, mL | 2.24 | 1.91 | 1.58 | 1.08 | 1.02 |
10% APS, µL | 25 | 25 | 25 | 25 | 20 |
TEMED, µL | 7 | 7 | 7 | 7 | 10 |
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl |
2 gels: | 8% | 10% | 12% | 15% | Stacking |
---|---|---|---|---|---|
3.5x buffer, mL | 2.84 | 2.84 | 2.84 | 2.84 | 1 |
30% acrylamide, mL | 2.68 | 3.34 | 4 | 5 | 0.46 |
H2O, mL | 4.48 | 3.82 | 3.16 | 2.16 | 2.04 |
10% APS, µL | 50 | 50 | 50 | 50 | 40 |
TEMED, µL | 14 | 14 | 14 | 14 | 20 |
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl |
3 gels: | 8% | 10% | 12% | 15% | Stacking |
---|---|---|---|---|---|
3.5x buffer, mL | 4.26 | 4.26 | 4.26 | 4.26 | 1.5 |
30% acrylamide, mL | 4.02 | 5.01 | 6 | 7.5 | 0.69 |
H2O, mL | 6.72 | 5.73 | 4.74 | 3.24 | 3.06 |
10% APS, µL | 75 | 75 | 75 | 75 | 60 |
TEMED, µL | 21 | 21 | 21 | 21 | 30 |
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl |
4 gels: | 8% | 10% | 12% | 15% | Stacking |
---|---|---|---|---|---|
3.5x buffer, mL | 5.68 | 5.68 | 5.68 | 5.68 | 2 |
30% acrylamide, mL | 5.36 | 6.68 | 8 | 10 | 0.92 |
H2O, mL | 8.96 | 7.64 | 6.32 | 4.32 | 4.08 |
10% APS, µL | 100 | 100 | 100 | 100 | 80 |
TEMED, µL | 28 | 28 | 28 | 28 | 40 |
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl |