Pricing

Independent pricing represents a reasonable amount of supplies for fixation and embedding, including, slides, grids, stains and equipment usage. The Electron Microscopy Center provides four hours of training in electron microscopy techniques and use of the TEM. The cost for this training is charged to the researcher at the rate of $165/hour. Final decision on whether researcher can go on their own is determined by the lab manager.

Fifteen mls of a modified Karnovsky’s fixative can be provided for up to 10 specimens; additional fixatives or special types are available at an extra cost. Tissue specimens for TEM should be about 2 x 2 x 2mm or smaller. For special types of specimens (cultured cells, thin layers of tissue, tissue that requires special orientation), email Caroline Miller for instructions.

Outputs refer to direct digital images. Electron Microscopy film is not used in the Electron Microscopy Center.

Transmission Electron Microscopy

Full Service

 

Independent

 

Routine Specimen Preparation:
Fixation through Embedding1-2 Samples$375.00
3-10 Samples$475.00
10+ Samples+$50.00/each additional sample
Microtomy:
Thick Sectioning and Staining/blk$40.00/blk
Thin Sectioning and Staining/blk(for every 4 grids)$60.00/blk
Electron Microscopy:
per hour spent on scope$165.00$80.00
CD$4.00
Technical Time$85.00/hr

Special Procedures, Immunocytochemistry-EM level (no independent)

Full Service

 

Specimen Preparation:
Fixation-Embedding, Unicryl1-2 Samples$375.00
3-10 Samples$475.00
10+ Samples+$50.00/sample
Cryo Preparation:TBATBA
Microtomy:
Thick Sectioning and Staining/blk$45.00/blk
Thin Sectioning and Staining/blk8 grids$65.00/blk
Eight grids represents enough for two immuno attempts; a $5 charge per grid over eight applies.
Immunostaining at the TEM level, for each attempt & antibody****$450.00
Immunostaining at the LM level (on plastic thick sections), for each attempt and antibody$225.00
Negative Staining (per grid)$45.00
Results cannot be guaranteed, because antibody specificity/titer, quality/fixation of tissue and amount of target protein can limit success. Investigator provides primary antibody and necessary background literature.
Preparation of coated grids without negative staining$30.00

Scanning Electron Microscopy

Full Service

 

Independent

 

Routine Specimen Preparation*:
Fixation and Coating5 Samples$500.00
over 5 Samples+$45.00/each additional sample
Coating Only: $20.00/Sample
Electron Microscopy:
per hour spent on scope$165.00$80.00

Evaluation/Consultation:

Each incoming project receives an hour consultation at no charge, thereafter an $85 per hour charge applies for time spent researching techniques, evaluating data (quantitative and/or qualitative), or for anything beyond routine. The Electron Microscopy Center provides four hours of training in electron microscopy techniques or use of either the TEM or SEM. The cost for this training is charged to the investigator at the rate of $165/hour. If retraining is necessary, that is charged at the rate of $165.00/hr.

External/ Commercial Projects

Each external or commercial project is charged double the cost of regular fee schedule due to lack of subsidy that applies to IU School of Medicine projects.