Electron Microscopy Center

The Indiana University School of Medicine Electron Microscopy Center is a full-service research laboratory that offers both transmission electron microscopy and scanning electron microscopy. The center can provide technical services to help design and then implement experiments needing either type of microscopy.

Free consultation with the center’s manager or director is available as part of any new experiment. The service provided can apply both traditional methods and more recent technical developments to meet the investigator’s needs.

Full Service Support

The following procedures are provided as a full service with independent use allowed for use on the microscopes (TEM, Transmission Electron Microscope and SEM, Scanning Electron Microscope). Training is provided at the full-service price until capabilities on the equipment are confirmed. Independent work is billed at a lower cost (see fee schedule).

The Electron Microscopy Center Director has the authority to revoke the independent-use privilege or require retraining to ensure the safety of the Electron Microscopy Center equipment. All additional training requested or required are billed at the technician rate.

Standard routine processing of specimens with fixation, critical point or chemical drying and mounting. Viewing and photography on the SEM. Standard supplies (including some fixatives) are provided. Digital images provided on CD. Standard routine processing includes either tissue or cell cultures either as monolayers.

Standard routine processing of specimens with fixation through embedding. Standard thick and thin sectioning with staining. Viewing and photography on the TEM. Standard photography, digital images provided on CD. Standard supplies (including some fixatives) are provided. Standard routine processing includes either tissue or cell cultures either as monolayers, on membranes or as pellets and special orientation. The facility has the capability to do negative staining and rotary shadowing.

Standard routine processing of specimens with fixation and embedding in Unicryl. Standard routine thick and thin sectioning for post-embedding immunostaining. Immunostaining*. Viewing and photography on the TEM. Digital images provided on CD.

For immunostaining, the primary antibody is provided by the investigator; the Electron Microscopy Center provides the secondary and can do pre-embedding immunostaining if desired.

TEM Microscope

TEM Microscope

Tecnai G2 12 Bio Twin. FEI, Hillsboro, OR equipped with AMT CCD Camera (Advanced Microscopy Techniques, Danvers, MA)

SEM Microscope

SEM Microscope

JEOL 6390 LV (Peabody MA)

Ultramicrotomes

Ultramicrotomes

UCT Ultracut with FCS. FCS, Cryosectioning Attachment (Leica, Bannockburn, IL)

Critical Point Dryer

Critical Point Dryer

Samdri-790 (Tousimis, Rockville, MD)

Sputter Coater

Sputter Coater

Polaron (Energy Beam Sciences, East Gramby, CT)

Vibratome

Vibratome

Warner Instruments (Harvard Bioscience Company, Series 1000)