Faculty Research Labs

Bis-Tris Gel Recipes

1 gel:8%10%12%15%Stacking
3.5x buffer, mL1.421.421.421.420.5
30% acrylamide, mL1.341.6722.50.23
H2O, mL2.241.911.581.081.02
10% APS, µL2525252520
TEMED, µL777710
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

2 gels:8%10%12%15%Stacking
3.5x buffer, mL2.842.842.842.841
30% acrylamide, mL2.683.34450.46
H2O, mL4.483.823.162.162.04
10% APS, µL5050505040
TEMED, µL1414141420
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

3 gels:8%10%12%15%Stacking
3.5x buffer, mL4.264.264.264.261.5
30% acrylamide, mL4.025.0167.50.69
H2O, mL6.725.734.743.243.06
10% APS, µL7575757560
TEMED, µL2121212130
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl

 

4 gels:8%10%12%15%Stacking
3.5x buffer, mL5.685.685.685.682
30% acrylamide, mL5.366.688100.92
H2O, mL8.967.646.324.324.08
10% APS, µL10010010010080
TEMED, µL2828282840
3.5x buffer: 52.32g bis-tris in 200mL H2O, pH 6.5 to 6.8 with HCl