Heather A. Hundley, PhD
Associate Professor of Biochemistry & Molecular Biology
Trustee Teaching Award, IUSM
Travel Award, RNA Society
Women in Science Travel Award, IU Provost’s Office
Bergmann Prize, US-Israel Binational Science Foundation
American Cancer Society Research Scholar Award
American Cancer Society Institutional Research Grant, IU Simon Cancer Center
Ralph W. and Grace M. Showalter Research Trust Grant, IUSM
Biomedical Research Enhancement Grant, IUSM
Helen Hay Whitney Post-doctoral Fellowship, 2006-2009
My research program takes an integrated approach using C. elegans and human cell lines to understand the biological impact of RNA editing. RNA editing is a post-transcriptional modification that alters the nucleotide sequence of RNA from that encoded by the genome. The most prevalent type of editing in humans is adenosine (A) to inosine (I) editing, with ~1,000,000 editing events identified in the human transcriptome. Brain mRNAs, some of which are required for neurogenesis, are the most edited human transcripts, and deletion of the editing machinery in lower organisms, such as C. elegans, results in behavioral defects, indicating RNA editing is required for proper neuronal development and function. In addition, alterations in editing levels have been observed in a number of neuropathological diseases, including epilepsy, depression, amyotrophic lateral sclerosis, and brain tumors. Despite the biological importance of RNA editing, the consequences of editing on normal gene expression and the mechanistic implications of aberrant editing in disease are only beginning to be dissected.
Research in the Hundley lab utilizes a combination of biochemistry, genomics and molecular biology to both charaterize the effects of editing on gene expression and elucidate molecular mechanisms that are important for regulating RNA editing.We have recently utilized next generation sequencing and molecular biology approaches to identify a major regulator of noncoding editing in C. elegans. Current efforts in the Hundley lab are focused on dissecting the molecular mechanism of this regulator and determining the conservation of this regulatory protein in human cells.
Adenosine Deaminase That Acts on RNA 3 (ADAR3) Binding to Glutamate Receptor Subunit B Pre-mRNA Inhibits RNA Editing in Glioblastoma.
Trans and cis factors affecting A-to-I RNA editing efficiency of a noncoding editing target in C. elegans.
Noncoding regions of C. elegans mRNA undergo selective adenosine to inosine deamination and contain a small number of editing sites per transcript.
The dsRBP and inactive editor ADR-1 utilizes dsRNA binding to regulate A-to-I RNA editing across the C. elegans transcriptome.
Inverted Alu dsRNA structures do not affect localization but can alter translation efficiency of human mRNAs independent of RNA editing.
Dissecting functional similarities of ribosome-associated chaperones from Saccharomyces cerevisiae and Escherichia coli.
The in vivo function of the ribosome-associated Hsp70, Ssz1, does not require its putative peptide-binding domain.