The research lab of Johnathan D. Tune, PhD, focuses on the regulation of coronary blood flow, contractile function and metabolism in health and disease.
Research in the Tune Lab focuses on the regulation of coronary blood flow, contractile function and metabolism in health and disease. The laboratory has been continuously funded for more than 15 years by the National Institutes of Health, the American Heart Association and the American Diabetes Association as well as pharmaceutical companies to elucidate mechanisms of impaired coronary and cardiac function in the setting of obesity and diabetes. The lab’s studies routinely include a series of experimental approaches utilizing both in vivo (chronically instrumented conscious and/or acute open-chest swine) and in vitro (isolated artery rings, pressurized arterioles, Western blot, RT-PCR, immunohistochemistry, confocal microscopy) methodologies to examine hypotheses in a highly integrative manner. These studies have led to more than 95 peer-reviewed publications and the successful training of numerous post-doctoral fellows, PhD and MS students.