Protocols

Corson Lab protocols range from the routine to the esoteric best practices and tips. External research labs are invited to both use and share these protocols, with appropriate attribution to the Corson Lab at Indiana University School of Medicine.

Routine Cell Culture
Guidelines and general procedures are offered for culturing, subculturing and freezing adherent cells within a traditional lab setting.
Learn More Routine Cell Culture

Culture Cell Tips
Best practices are provided to avoid culture contamination for optimal results.
Learn More Culture Cell Tips

Bis-Tris Gel Recipes
Recipes for bis-tris polyacrylamide gels are designed for optimal separation of a wide range of proteins.
Learn More Bis-Tris Gel Recipes

SDS-PAGE and ImmunoblottingVertical CTA 3
Scientists can determine characteristics of protein antigens within complex mixtures using Laemmli and bis-tris protocols.
Learn More SDS-PAGE and ImmunoblottingVertical CTA 3

Soft Agar Colony Assay
Scientists can confirm cellular anchorage-independent growth using the soft agar colony assay method.
Learn More Soft Agar Colony Assay

Whole-Cell Pulldown Protocol
An alternative method to whole-cell pulldown involves cell fractionation using biotinylated affinity reagents.
Learn More Whole-Cell Pulldown Protocol

Silver Staining
Best practices are outlined to achieve optimal results for mass spectrometry compatible silver staining.
Learn More Silver Staining

Precautions for Working with RNA 
Due to its liable nature within the lab, simple precautions reduce the degradation of RNA samples.
Learn More Precautions for Working with RNA 

Publication for Powerpoint
Simple instructions are provided to enhance high-resolution graphic files for publications and presentations.
Learn More Publication for Powerpoint

Corson Lab

Publications