Tele: (317) 274-5287
Email: bmolitor@iupui.edu

PubMed Listing

Affiliations:

Washington University School of Medicine, St. Louis, Missouri
University of Colorado School of Medicine, Denver, Colorado
University of Colorado School of Medicine, Denver, Colorado
University of Colorado School of Medicine, Denver, Colorado
Indiana University, Indianapolis, Indiana

Research Interests:

My research interests have centered around the cell biology of acute renal failure with an emphasis on proximal tubule cell injury secondary to ischemia or nephrotoxins. Studies have utilized both in vivo and in vitro cell culture techniques to understand plasma membrane and actin cytoskeletal interactions and alterations. We have documented rapid duration-dependent loss of surface membrane lipid and protein polarity, opening of cellular tight junctions and polymerization and redistribution of the actin cytoskeleton during ischemia.

We are now pursuing the mechanisms responsible for these alterations. A second area of study within the laboratory involves the pathophysiology of nephrotoxin, primarily aminoglycosides, induced cellular injury. Questions being asked include; How does gentamicin traffic within the cell to subcellular organelles? How does gentamicin induce cellular toxicity? How do cells adapt to continuous gentamicin administration? Specific techniques employed include confocal microscopy, multi photon microscopy, fluorescent analogue cytochemistry, cell microinjection, immunocytochemical, immunogold and quantitative biochemical techniques including protein isolation, characterization and western blotting.